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iscript advanced cdna conversion kit  (Bio-Rad)


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    Bio-Rad iscript advanced cdna conversion kit
    Iscript Advanced Cdna Conversion Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iscript advanced cdna conversion kit/product/Bio-Rad
    Average 94 stars, based on 45 article reviews
    iscript advanced cdna conversion kit - by Bioz Stars, 2026-05
    94/100 stars

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    The boxplots compare (A) nucleic acid yield, (B) total raw reads, and (C) median read length between DNA- and RNA-based sequencing libraries. Histograms show the sequencing read length distribution for all (D) DNA, and (E) cDNA reads, with dashed vertical lines indicating the median read lengths. (F) Number of total metagenomic reads after filtering and subset of reads mapping to the phylum and genus levels and to the top 20 genera. The horizontal dashed line indicates the downsampling threshold of 87,000 reads. GA3.1 was excluded for subequent analyses since it did not reach the downsampling threshold.

    Journal: bioRxiv

    Article Title: Real-time genomic pathogen, resistance, and host range characterization from passive water sampling of wetland ecosystems

    doi: 10.1101/2025.09.05.674394

    Figure Lengend Snippet: The boxplots compare (A) nucleic acid yield, (B) total raw reads, and (C) median read length between DNA- and RNA-based sequencing libraries. Histograms show the sequencing read length distribution for all (D) DNA, and (E) cDNA reads, with dashed vertical lines indicating the median read lengths. (F) Number of total metagenomic reads after filtering and subset of reads mapping to the phylum and genus levels and to the top 20 genera. The horizontal dashed line indicates the downsampling threshold of 87,000 reads. GA3.1 was excluded for subequent analyses since it did not reach the downsampling threshold.

    Article Snippet: Whole-genome AIV sequencing of positive samples was performed after complementary DNA (cDNA) conversion and multi-segment amplification using M-RTPCR , targeting conserved regions across all AIV segments: Briefly, the RNA was mixed with SuperScript III One-Step PCR (Invitrogen) buffer and enzyme mix (containing the reverse transcriptase enzyme and the PCR amplification enzyme).

    Techniques: RNA Sequencing, Sequencing